Fig. 3.

Hyperbiofilm variants prevail in biofilm competitions. A–F The competition of ancestor and hyperbiofilm variants in planktonic cultures and biofilms when inoculated at the same ratio (A–C) or ancestral: hyperbiofilm mutant = 5:1 (D–F). G Disc diffusion antibiotic sensitivity testing, H growth curves measurement, and I biofilm growth curve was measured. Data are presented as the mean ± s.d. of four biological replicates. Significance was determined using a Student’s t test: n.s indicates no significant difference (P ≥ 0.05); ***P < 0.001