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. 2021 Jul 7;12:693983. doi: 10.3389/fphar.2021.693983

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Copyright © 2021 Zheng, Zhuo, Nie, Liu, Chen, Wu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of POL, ACT, and FTB on cell apoptosis rate, ROS levels, apoptosis-related genes and inflammatory factors (A,B) Effects of POL, ACT, and FTB on TNF-α-induced apoptosis were evaluated using flow cytometry (C–E) Relative mRNA expression levels of Caspase 3, Caspase 8, and Caspase 9 (F) Effects of POL, ACT, and FTB on TNF-α-induced ROS levels were observed using a fluorescence microscope. Scale bar indicates 100 μm (G–I) Effects of POL, ACT, and FTB on TNF-α-induced expression levels of IL-8, IL-1β, and IL-6 were determined using ELISA kits. Values represent the means ± SD (n = 3-6). ^## p < 0.01 vs control, **p < 0.01 vs TNF-α.