Figure 5.

Single-cell analysis of PDGFRβ⁺ bone cells

(A) Experimental strategy for scRNA-seq analysis of Pdgfrb-CreERT2-labeled (GFP⁺) cells.

(B and C) t-SNE plot with color-coded cell clusters in scRNA-seq analysis of GFP⁺ cells. Clusters represent chondrocytes (CHO1, CHO2, and CHO3), smooth muscle cells (SMCs), mpMSCs and dpMSCs, a small population of GFP⁺ hematopoietic cells (HCs), osteoblastic cells (OBs), and fibroblasts (FBs) (B). Feature plots showing GFP transcripts in all clusters and expression of cell-type-specific marker genes (green) (C).

(D and E) Heatmap displaying expression of selected cell-type-specific mark genes (D). Violin plots showing expression of selected markers in cell subclusters (E).

(F and G) Monocle trajectory analysis of color-coded Pdgfrb-CreERT2-labeled clusters (F). Differentiation pathway of each cell cluster (G).

See also Figure S6