REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat monoclonal anti-Endomucin (V.7C7)	Santa Cruz	Cat# sc-65495; RRID: AB_2100037
Rabbit polyclonal anti-Acan	Millipore	Cat#AB1031; RRID: AB_90460
Goat anti-Pdgfrb	R & D	Cat# AF1042; RRID: AB_2162633
Rabbit polyclonal anti-Osterix/sp7	Abcamabcam	Cat# ab22552 RRID: AB_2194492
Rabbit monoclonal anti-Runx2	Abcam	Cat# ab22552; RRID: AB_2713945
Mouse monoclonal anti-alpha-smooth muscle actin (Cy3 - conjugated)	Sigma-Aldrich	Cat#C6198; RRID: AB_476856
Rabbit polyclonal anti-Ki67	Abcam	Cat#ab15580; RRID: AB_443209
Chicken polyclonal anti-GFP	Abcam	Cat#ab13970; RRID: AB_300798
Goat anti-Pdgfra	R&D	Cat#AF1062; RRID: AB_2236897
Goat polyclonal anti-CD31	R&D	Cat#AF3628; RRID: AB_2161028
Rabbit monoclonal anti-Perilipin	Cell signaling	Cat#9349; RRID: AB_10829911
Goat polyclonal anti-Esm1	R&D	Cat# AF1810; RRID: AB_2246741
Goat polyclonal anti-Osteopontin	R&D	Cat# AF808; RRID: AB_2194992
Rabbit polyclonal anti-Osteocalcin	Lifespan Bioscience	Cat#LS-C17044; RRID: AB_798874
Rabbit polyclonal anti-Collagen IV	AbD Serotec	Cat# 2150-1470; RRID: AB_2082660
Rabbit polyclonal anti-Collagen IIIA1	Abcam	Cat# ab7778; RRID: AB_306066
Rabbit monoclonal anti-JunB	Cell signaling	Cat#3753; RRID: AB_2130002
Anti rat Alexa Fluor 488	ThermoFisher Scientific	Cat#A21208 RRID: AB_141709
Anti rat Alexa Fluor 594	ThermoFisher Scientific	Cat#A21209 RRID: AB_2535795
Anti goat Alexa Fluor 546	ThermoFisher Scientific	Cat#A11056 RRID: AB_142628
Anti goat Alexa Fluor 647	ThermoFisher Scientific	Cat#A21447 RRID: AB_141844
Anti rabbit Alexa Fluor 647	ThermoFisher Scientific	Cat#A31573 RRID: AB_2536183
Anti rabbit Alexa Fluor 488	ThermoFisher Scientific	Cat#A21206 RRID: AB_141708
Anti chicken Alexa Fluor 488	Jackson Laboratories	Cat#703-545-155; RRID: AB_2340375
Chemicals, peptides, and recombinant proteins
Tamoxifen	Sigma	Cat#T5648
4’,6’-diamidino-2-phenylindole (DAPI)	Sigma	Cat#D9542
Kolliphor EL	Sigma	Cat#C5135
Critical commercial assays
lineage cell depletion kit	MACS	Cat#130-090-858
CD45 MicroBeads	MACS	Cat#130-052-301
CD117 MicroBeads	MACS	Cat#130-091-224
RNeasy Plus Mini Kit	QIAGEN	Cat# 74134
iScript cDNA Synthesis kit	BIO-RAD	Cat# 1708890
TaqMan Gene Expression Master Mix	ThermoFisher Scientific	Cat#4369016
PowerUp SYBR Green Master Mix	ThermoFisher Scientific	Cat#A25742
Quantification-RNA Qubit Fluorometric quantitation	ThermoFisher Scientific	Cat#Q32851
High sensitive DNA kit	Agilent Bioanalyzer	Cat#5067-4626
Chromium Single cell 3′ Reagent Kit v2	10X genomics	Cat#PN-120237
NextSeq 500/550 High Output Kit V2 (150 cycles)	Illumina	Cat#20024907
NextSeq 500/550 Mid Output Kit V2	llumina	Cat#20024904
Deposited data
Bulk-RNA seq data mpMSC and dpMSC	This paper	GSE156636:GSE156634
single cell-RNA seq data BMSCs	This paper	GSE156636:GSE156635
single cell-RNA seq data -Pdgfrb-Cre GFP+ cells	This paper	GSE156636:GSE156635
Experimental models: Cell lines
Primary mouse bone MSC	This paper	N/A
Experimental models: Organisms/strains
C57/BL6	Janvier Labs	N/A
Gt(Rosa26) ACTB-tdTomato-EGFP	The Jackson Laboratory	JAX: 007676
Pdgfra-H2B-GFP	The Jackson Laboratory	JAX:007669
Hey1GFP	Gensat
Cspg4-DsRed	The Jackson Laboratory	JAX:008241
Sp7 (Osterix)-mCherry	The Jackson Laboratory	JAX:024850
Pdgfrb(BAC) CreERT2	Laboratory of Ralf H.Adams	(Kato et al., 2018)
Myh11(BAC)Cre ERT2	The Jackson Laboratory	JAX:019079
Rosa26-hPDGFB-GOF	Laboratory of Christer Betsholtz	(Armulik et al., 2010)
Cdh5(PAC) Cre-ERT2	Laboratory of Ralf H.Adams	(Sivaraj et al., 2020)
Leprtm2(cre)Rck/J	The Jackson Laboratory	JAX:008320
Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J	The Jackson Laboratory	JAX:007908
Lepr(BAC) Cre-ERT2	This paper
Rosa26-DTA	The Jackson Laboratory	JAX:010527
Gli1-CreERT2	The Jackson Laboratory	JAX:007913
Junbflox/flox	The Jackson Laboratory	JAX:012369
Oligonucleotides
Eukaryotic 18S rRNA	ThermoFisher Scientific	Cat#4319413E
Pdgfrb (Mm00435546_m1)	ThermoFisher Scientific	Cat#4331182
Pdgfra (Mm00440701_m1)	ThermoFisher Scientific	Cat# 4331182
Sp7 (Mm04209856_m1)	ThermoFisher Scientific	Cat# 4331182
Alpl (Mm00475834_m1)	ThermoFisher Scientific	Cat# 4331182
Spp1(Mm00436767_m1)	ThermoFisher Scientific	Cat# 4331182
Bglap (Mm03413826_m1)	ThermoFisher Scientific	Cat#4331182
Runx2 (Mm00501584_m1)	ThermoFisher Scientific	Cat# 4331182
Adipoq (Mm00456425_m1)	ThermoFisher Scientific	Cat# 4331182
Cfd (Mm01143935_m1)	ThermoFisher Scientific	Cat#4331182
Pparg (Mm00440940_m1)	ThermoFisher Scientific	Cat# 4331182
Acan (Mm00545794_m1)	ThermoFisher Scientific	Cat#4331182
Col11a2 (Mm00487046_m1)	ThermoFisher Scientific	Cat# 4331182
Software and algorithms
Volocity 6.3 (image analysis)	PerkinElmer	N/A
FACS software, FlowJo	FLOWJO, LLC	N/A
GraphPad Prism 7	GraphPad Software, inc	N/A
Adobe illustrator	Adobe	N/A
Adobe Photoshop	Adobe	N/A