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. 2021 Jul 7;12:687394. doi: 10.3389/fphar.2021.687394

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Copyright © 2021 Liu, Xu, Cheng, Zhou, Zhou, He, Hu, Zhang, Chen and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GN combination ameliorated H₂O₂-induced apoptosis in HUVECs. (A) The concentration of GSH in cell supernatants. (B) The concentration of SOD in cell supernatants. (C) The concentration of MDA in cell supernatants. (D) Representative images of cellular apoptosis detected by flow cytometry. (E) ROS generation induced by H₂O₂ in HUVECs on fluorescence microscopy (scale bar: 1,000 μm, 100×). (F) The expression of NOX2, p22phox and GAPDH in HUVECs was determined by WB. (G) The relative protein levels of NOX2 and p22phox were normalized to GAPDH. (H) The expression of Bax, Bcl2, caspase-3 and GAPDH in HUVECs was determined by WB. (I) The relative protein levels of caspase-3, Bax and Bcl2 were normalized to GAPDH. All the data are presented as the mean ± SD, n = 3. *p < 0.05 vs. control group, **p < 0.01 vs. control group, ^# p < 0.05 vs. model group, ^## p < 0.01 vs. model group.