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. 2021 Jul 7;9:661317. doi: 10.3389/fcell.2021.661317

FIGURE 2.

FIGURE 2

IRP2 silencing attenuated hemin-induced ferroptosis in primary neurons in vitro. (A) Cell viability examined MTT assay, (B) western blot analysis of IRP2, TfR1, and FPN, and (C) levels of iron, MDA, GSH, and GPX4 in the primary cortical neurons which were treated with hemin (100 μM) or saline for 24 h. (D) Relative IRP2 mRNA level determined by qRT-PCR, (E) cell viability examined MTT assay, (F) western blot analysis of TfR1 and FPN, and (G) levels of iron, MDA, GSH, and GPX4 in the primary cortical neurons which were transfected with sh-IRP2 or sh-NC and treated with hemin (100 μM) for 24 h. The data are presented as the mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, vs. Control or Hemin+sh-NC.