Figure 2.

Workflow of single-cell RNA sequencing analysis of mouse and human teeth. Teeth are excised/isolated (1, 2), and dental tissues are dissociated into single-cell suspensions (3). Cell of interest may be sorted by a fluorescent activated cell sorter (FACS) (4) or directly examined for live cells. Cells are then individually barcoded (5) before complementary DNA libraries are prepared and sequenced. The resulting files undergo rounds of quality control and filtering (6). Cells can then be grouped based on the similarity of their transcriptomes through unsupervised clustering (7), and markers are used to identify cell types in the data set (8). Created with BioRender.com.