Spectral properties of PhoCl variants^a.

Protein	Extinction coefficientsb,c (mM−1 cm−1)		Quantum yieldb 488 nm	Brightness 488 nm (mM−1 cm−1)	Dissociation half times (s)	Dissociation efficiencyd (%)	Reference
	ε (488 nm)	ε (405 nm)
mMaple	22 ± 0	11 ± 2	0.89	20	NA	NA	34
PhoCl1	34 ± 0	8.9 ± 0.8	0.73	25	570	71	5
PhoCl2c	24 ± 3	11 ± 1	0.48	12	700	92	This work
PhoCl2f	26 ± 2	12 ± 1	0.50	13	76	88	This work
PhoCl1.5f-MV	30 ± 4	12 ± 2	0.53	16	130	90	This work
PhoCl1.5f-L	26 ± 2	12 ± 0	0.45	12	140	79	This work
PhoCl1.5f-MVS	25 ± 0	13 ± 0	0.29	7.3	30	93	This work
PhoCl1.5f-LS	22 ± 3	13 ± 3	0.36	7.9	21	85	This work
PhoCl1.5f-MVLS	NA	NA	NA	NA	14	NA	This work

^aAll measurement were performed with purified proteins in PBS solution (pH 7.4).

^bEGFP extinction coefficient of 56 mM⁻¹ cm⁻¹ (ref. 36) and quantum yield of 0.6 (ref. 37) at 488 nm were used as reference standards.

^cMean ± SD for each protein was determined via alkali denaturation method from two independent protein preparations.

^dDissociation efficiencies listed in this table were characterized by gel filtration chromatography (GFC) assay.