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. 2021 Jul 7;9:691560. doi: 10.3389/fcell.2021.691560

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Copyright © 2021 Bender, Fietz, Richter and Stanojlovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of the p-α-syn in the hippocampus of the 6 and 16-month-old WT and Thy1-aSyn mice. Double-immunofluorescence for p-α-syn (green) and NeuN (orange) and DAPI staining (blue) and merged images of 6 and 16 months-old wildtype [WT; (A,B)], and 6 and 16 months-old Thy1-aSyn [asyn; (C,D)] mice. Cornu ammonis (CA1, CA2, and CA3), dentate gyrus (DG) regions. Scale bars, 200 μm. High magnification Apotome images (63×, oil immersion), double-immunofluorescence for p-α-syn (green) and NeuN (orange) and DAPI staining (blue) and merged images of Thy1-aSyn mice (E,F). CA1so, CA1 stratum oriens; CA1sr, CA1 stratum radiatum; CA1sp, CA1 pyramidal layer (E), GCL, DG granule cell layer; DGmo, DG molecular layer; PL, DG polymorph year (F). Arrows indicate NeuN positive cells and p-α-syn accumulations. Scale bar 50 μm.