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. 2021 Jul 7;9:691560. doi: 10.3389/fcell.2021.691560

FIGURE 2.

FIGURE 2

Quantification of PCNA, Pax6 and Tbr2 positive cells in the hippocampal dentate gyrus of wildtype and Thy1-aSyn mice. Double-immunofluorescence for PCNA (green) and Pax6 (red) and DAPI staining (blue) and merged images of 6-month-old wildtype [WT, (A)] and Thy1-aSyn [asyn, (B)] mice. Number of PCNA+ cells (C), Pax6+/PCNA+ cells (D) in the granule cell layer (GCL) and subgranular zone (SGZ) of 6- and 16-month (mo)-old wildtype (WT) and Thy1-aSyn (asyn) mice. Double-immunofluorescence for PCNA (green) and Tbr2 (red) and DAPI staining (blue) and merged image of 6-month-old wildtype [WT, (E)] and Thy1-aSyn [asyn, (F)] mice. Number of Tbr2 + /PCNA + cells (G) in the GCL and SGZ of 6 and 16 month-old (WT) and Thy1-aSyn (asyn) mice. Data represent mean ± SEM. (WT; 6 month-old, n = 6, 16 month-old n = 6; asyn, 6 month-old, n = 6, 16 month-old n = 5; Student’s t-test; ∗p < 0.05, ∗∗∗p < 0.005). GCL, granule cell layer; ML, molecular layer; SGZ, subgranular zone; PL, polymorphic layer. Scale bars, 50 μm.