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. 2021 Jul 7;9:676409. doi: 10.3389/fbioe.2021.676409

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Copyright © 2021 Dong, Song, Liu, Yu, Shen, Yang, Yang, Hu, Zhang and Gu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biocompatibility test of biomimetic scaffolds. Cytotoxicity of PNIPAAm-b-PEG of different concentrations were detected by CCK-8 method on (A) days 1, (B) 3, and (C) 7. (D) Cell viability was assessed by live/dead staining of ADSCs after culture of 5 days, in which live cells were stained in green and dead cells were stained in red (Scale bar = 100 μm). (E) Cellular morphology of ADSCs (in red arrows) seeded in scaffolds was observed via scanning electron microscopy after 1, 3, and 5 days of culture. *P < 0.05 vs. the control group.