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. 2021 Jul 7;12:705575. doi: 10.3389/fphys.2021.705575

TABLE 3.

Sigmar1 dependent regulation of ion channels through protein-protein interaction.

Ion channel Cell types Method of interaction detection Sigmar1 construct Ion Channel construct Expression References
Inositol tri-phosphate receptor (IP3R) NG-108, CHO, bovine brain mitochondria Co-IP Native Sigmar1 and Sigmar1-EGFP Native IP3R3 Native expression and transient expression Hayashi and Su, 2001, 2007; Natsvlishvili et al., 2015
NG-108 Co-IP and PLA Native Sigmar1 Native IP3R1 Native expression Kubickova et al., 2018
Rat heart tissue Co-IP Native Sigmar1 Native IP3R2 Native expression Tagashira et al., 2013a
Ryanodine receptor Rat heart tissue Co-IP Native Sigmar1 Native RYR2 Native expression Tagashira et al., 2013a
L-ype Voltage-gated calcium channel (VGCC) RGC-5 cells Co-IP Wild-type Sigmar1 Native L-type VGCC Stable and native expression Tchedre et al., 2008
Voltage-dependent N-type calcium channel (Cav2.2) HEK293T FRET and Co-IP Sigmar1-dsred EGFP-Cav2.2 Transient overexpression Zhang et al., 2017c
Calcium release-activated calcium channel tSA-201 Co-IP Sigmar1-FLAG ORAI-Myc Transient overexpression Srivats et al., 2016
Voltage-dependent potassium channel Mouse nucleus accumbens lysate, NG108-15 Co-IP and Co-IP with cross-linking Native Sigmar1 and Sigmar1-V5-His Native Kv1.2, wild-type Kv1.2 Native expression and transient expression Kourrich et al., 2013
HEK293 Co-IP Sigmar1-FLAG Kv1.3-HA Transient expression Kinoshita et al., 2012
Rat posterior pituitary lysate Co-IP Native Sigmar1 Native Kv1.4 Native expression Aydar et al., 2002
SK3 channel SKmel28 cells, HEK293 Co-IP and HTRF Sigmar1-Myc, HALO-Sigmar1-Myc Wild-type SK3, SK3-HA Transient and stable overexpression Gueguinou et al., 2017
Nav1.5 Na+ channel tSA201, MDA-MB-468, MDA-MB-231 Anti-FLAG chromatography, PLA, Co-IP Sigmar1-FLAG, Native Sigmar1 Nav1.5-HA, Native Nav1.5 Transient overexpression, native expression Balasuriya et al., 2012
Voltage-dependent anion channel 2 (VDAC2) MA-10 Co-IP Native Sigmar1 Native VDAC2 Native expression Marriott et al., 2012
Acid sensing ion channel 1a (ASIC1a) HEK293 Ni affinity chromatography Sigmar1-FLAG-His ASIC1a-His Stable overexpression (ASIC1a) and transient overexpression (Sigmar1) Carnally et al., 2010

Co-IP, Co-immunoprecipitation; PLA, proximity ligation assay; FRET, fluorescence resonance energy transfer; HTRF, homogeneous time resolved fluorescence.