Fig. 4. Bio-panning against the NODAL protein. (A) A scheme of three-rounds panning against NODAL and negative controls. (B) The top 20 sequences from the deep sequencing results were clustered into 4 groups and 6 of them were chemically synthesized. (C) Percentage of the phage recovery after each round of bio-panning. (D) Western blot validating with p-SMAD2 in response to treatment with rhNODAL and inhibitors in P19 cells. Total SMAD used as control (E) cell viability assay of TYK-nu cell line transfected with rhNODAL or GFP and treated with 19b at various peptide concentrations for 72 hours.
