Scenario	Description of the Scenario	Clinical guidelines	Laboratory guidelines
1st	To assess the effectiveness of disease‐specific sampling procedures of animals of listed species in a suspected establishment, based on clinical examination (TOR1.1) and laboratory examination (TOR1.2), in their ability to detect a category A disease in kept animals if the disease is present in that establishment, or to rule it out if not present (Art. 6 (2)).	Annex, CHAPTER IV.A.: 2. When an official veterinarian visits a suspected holding to confirm or rule out classical swine fever: – a check of the production and health records of the holding must be carried out, if these records are available; – an inspection in each subunit of the holding must be carried out to select the pigs to be clinically examined. The clinical examination must include the taking of body temperature and must primarily concern the following pigs or group of pigs: – sick or anorexic pigs; – pigs recently recovered from disease; – pigs recently introduced from confirmed outbreaks or from other suspected sources; – pigs kept in subunits recently visited by external visitors which had a recent close contact with classical swine fever suspected or infected pigs or for which other particularly risky contacts with a potential source of classical swine fever virus have been identified; – pigs already sampled and serologically tested for classical swine fever, in case the results of these tests do not allow to rule out classical swine fever, and in‐contact pigs. If the inspection in the suspected holding has not indicated the presence of the pigs or group of pigs referred to in the above subparagraph, the competent authority, without prejudice to other measures that may be applied in the holding in question in accordance with Directive 2001/89/EC and taking into account the epidemiological situation, shall: – carry out further examinations in the holding in question in accordance with subparagraph 3 below; 3. When reference is made to this paragraph, the clinical examination in the holding in question must be carried out on pigs selected at random in the subunits for which a risk of introduction of classical swine fever virus has been identified or is suspected. The minimum number of pigs to be examined must allow for the detection of fever if it occurs at a prevalence of 10% with 95% confidence in these subunits. However, in case of: – breeding sows: the minimum number of sows to be examined must allow for the detection of fever if it occurs at a prevalence of 5% with 95% confidence; – at semen collection centres: all boars must be examined. 4. If dead or moribund pigs are detected in a suspected holding, post‐mortem examinations must be carried out, preferably on at least five of these pigs and in particular on pigs: – that before death have shown or are showing very evident signs of disease; – with high fever; – recently dead. If these examinations have not shown lesions suggesting classical swine fever but, due to the epidemiological situation, further investigations are deemed necessary: – a clinical examination, as laid down in subparagraph 3 and blood sampling as laid down in subparagraph 5 must be carried out in the subunit where the dead or moribund pigs were kept; and – post‐mortem examinations may be carried on 3–4 in‐contact pigs. Emergency animal diseases: A field guide for Australian veterinarians (Department of Agriculture and CSIRO, 2019 ): CSF is an extremely variable disease and you cannot diagnose it based on clinical signs and gross pathology alone. Submit at least five pigs for post‐mortem examination, as individual animals may present with great variability in lesions. Record a composite picture of all lesions seen. Specific Domestic Animal Infectious Disease Quarantine Guidelines on Classical Swine Fever: Classical Swine Fever Diagnostics Manual (Ministry of Agriculture, Forestry and Fisheries of Japan, 2013 ): In case of suspicion, the prefectural animal health inspector shall thoroughly conduct clinical tests on abnormal pigs and pigs, etc. living together including measuring body temperatures. On this occasion, he/she shall record the status of a group of pigs, etc. including abnormal pigs.	Annex, CHAPTER IV.A.: 4. If dead or moribund pigs are detected in a suspected holding, post‐mortem examinations must be carried out, preferably on at least five of these pigs and in particular on pigs: — that before death have shown or are showing very evident signs of disease; — with high fever; — recently dead. If these examinations have not shown lesions suggesting classical swine fever but, due to the epidemiological situation, further investigations are deemed necessary: — a clinical examination, as laid down in subparagraph 3 and blood sampling as laid down in subparagraph 5 must be carried out in the subunit where the dead or moribund pigs were kept; and — post‐mortem examinations may be carried on 3–4 in‐contact pigs. Irrespective of the presence or absence of lesions suggesting classical swine fever, samples of the organs or tissues from pigs that have been subjected to post‐mortem examination must be collected for virological tests in accordance with Chapter V B. 1 These samples must be preferably collected from recently dead pigs. 5. If further clinical signs or lesions that may suggest classical swine fever are detected in a suspected holding, but the competent authority deems that these findings are not sufficient to confirm an outbreak of classical swine fever and that laboratory tests are therefore necessary, blood samples for laboratory tests must be taken from the suspected pigs and from other pigs in each subunit in which the suspected pigs are kept, in accordance with the procedures laid down below. The minimum number of samples to be taken for serological tests must allow for the detection of 10% seroprevalence with 95% confidence in the subunit in question. However, in the case of: – breeding sows: the minimum number of sows to be sampled must allow for the detection of 5% seroprevalence with 95% confidence (1); – a semen collection centre: blood samples must be taken from all boars. The number of samples to be taken for virological tests will be in accordance with the instructions of the competent authority, which will take into account the range of tests that can be performed, the sensitivity of the laboratory tests that will be used and the epidemiological situation. 6. If the suspicion of classical swine fever in the holding in question is related to the results of previous serological tests, in addition to the blood samples to be taken from the pigs referred to in 2, second subparagraph, fifth indent (i.e. pigs already sampled and serologically tested for classical swine fever, in case the results of these tests do not allow to rule out classical swine fever, and in‐contact pigs), the following procedures shall be applied: a) if the seropositive pigs are pregnant sows, some of them, preferably not less than three, shall be euthanised and subjected to a post‐mortem examination. Prior to killing a blood sample must be taken for further serological tests. The fetuses shall be subjected to examination for classical swine fever virus, virus antigen or virus genome in accordance with Chapter VI to detect intrauterine infection; b) if the seropositive pigs are sows with suckling piglets, blood samples must be taken from all piglets and shall be subjected to examination for classical swine fever virus, virus antigen or virus genome as referred to in Chapter VI. Blood samples must also be taken from the sows for further serological tests. 7. If, after the examination carried out in a suspected holding, clinical signs or lesions suggestive of classical swine fever are not detected, but further laboratory tests are deemed necessary by the competent authority to rule out classical swine fever, the sampling procedures laid down in subparagraph 5 shall be used for guidance purposes. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, heading ‘B. Diagnostic techniques’ (OIE, 2019a ): The variability of the clinical signs and post‐mortem lesions does not provide firm evidence for unequivocal diagnosis. tentative diagnosis based on clinical signs and post‐mortem lesions must therefore be confirmed by laboratory investigations. As pyrexia is one of the first signs of CSF and is accompanied by viraemia (Depner et al., 1994),detection of virus or viral nucleic acid in whole blood, collected in heparin or ethylene diamine tetra‐acetic acid (EDTA), or in tissues, collected from febrile animals, is the method of choice for detecting infected herds at an early stage. Targeted and risk‐based sampling should be performed, random sampling only being applied in cases where no clinical signs of disease are present. To increase the sensitivity of detection of virus, viral antigen or nucleic acid, clinically diseased animals and febrile animals should primarily be sampled. The Foreign Animal Disease Preparedness and Response Plan CLASSICAL SWINE FEVER STANDARD OPERATING PROCEDURES: 3. SURVEILLANCE. Appendix  B : Classical Swine Fever (CSF) Surveillance Plan (USDA, 2013 ).: When the Foreign Animal Disease Diagnostician (FADD) concurs that the herd meets the clinical case definition for CSF, the FADD will collect specimens for shipment to FADDL. At a minimum, specimens to be collected from live affected swine are serum, whole blood (EDTA or heparin), tonsil scrapings and nasal swabs. When possible, at least one pig, and ideally five pigs, should be posted and the following tissues collected: tonsil, lymph nodes, spleen, kidney and distal ileum. Emergency animal diseases: A field guide for Australian veterinarians (Department of Agriculture and CSIRO, 2019 ): CSF is an extremely variable disease and you cannot diagnose it based on clinical signs and gross pathology alone. Collect samples from affected, especially pyrexic, pigs killed immediately before a post‐mortem examination and from pigs that have recently died (including stillborn piglets and aborted fetuses). Collect: – serum, 30 samples from suspected chronically infected animals EDTA blood (7–10 ml/animal) from live, clinically affected animals – fresh tissue from the spleen, gastro‐hepatic lymph node, mesenteric lymph node, tonsils, lung, kidney and ileum (2 g of each tissue) – fixed tissue, a full range of tissues (including the brain) in neutral‐buffered formalin. The most rapid, sensitive and specific diagnostic procedure is the detection of viral nucleic acid in blood or in lymphoid tissue by qRT‐PCR. Specific Domestic Animal Infectious Disease Quarantine Guidelines on Classical Swine Fever: Classical Swine Fever Diagnostics Manual (Ministry of Agriculture, Forestry and Fisheries of Japan, 2013): Blood shall be collected from pigs, etc., which show symptoms and pigs, etc. living together (blood serum and blood with anticoagulant added) and be carried with carcasses of pigs, etc. or pigs, etc., which are raising suspicions of infection of classical swine fever or African swine fever, to a livestock hygiene service centre. A livestock hygiene service centre shall collect samples (including amygdalae, kidney and spleen without fail) necessary for the pathological appraisal (classical swine fever and differential diagnosis) from the carcasses of pigs, etc. or pigs, etc., which are raising suspicions of infection of classical swine fever or African swine fever.
2nd	To assess the effectiveness of disease‐specific sampling procedures, based on laboratory examination (ToR1.2), in their ability to detect the disease in the event of preventive killing, and in their ability to support with the epidemiological investigation (disease detection, prevalence estimation, virus identification, etc.) in kept animals of listed species in an affected establishment, before or when they are killed or found dead. The purposes of the epidemiological enquiry are described in Article 57 of Regulation (EU)2016/429.	NA	Annex, CHAPTER IV.C.: 1. In order that classical swine fever may be confirmed or ruled out and additional epidemiological information is gained, when pigs are killed as a preventive measure on a suspected holding in accordance with the provisions of Article 4(3)(a) or Article 7(2) of Directive 2001/89/EC, blood samples for serological tests as well as blood or tonsils samples for virological tests must be taken in accordance with the procedure laid down in subparagraph 2. 2. Sampling must primarily concern: – pigs showing signs or post‐mortem lesions suggesting classical swine fever and their in‐contact pigs; – other pigs which might have had risky contacts with infected or suspected pigs or which are suspected to have been contaminated with classical swine fever virus. These pigs must be sampled in accordance with the instructions of the competent authority, which will take into account the epidemiological situation. In this case, the sampling procedures laid down in the second, third and fourth subparagraphs below shall be used for guidance purposes. Furthermore, pigs proceeding from each of the subunits of the holding must be sampled at random. In this case, the minimum number of samples to be taken for serological tests must allow for the detection of 10% seroprevalence with 95% confidence in the subunit in question. However, in the case of: ‐breeding sows: the minimum number of sows to be sampled must allow for the detection of 5% seroprevalence with 95% confidence; In certain cases, e.g. when classical swine fever is suspected in a holding with a limited number of young pigs, the proportion of infected sows may be very small. In these cases a higher number of sows must be sampled. – a semen collection centre: blood samples must be taken from all boars. The type of samples to be taken for virological tests and the test to be used will be in accordance with the instructions of the competent authority, which will take into account the range of tests that can be performed, the sensitivity of these tests and the epidemiological situation. Annex, CHAPTER IV.B.: 1. In order that the manner of introduction of classical swine fever virus into an infected holding and the length of time elapsed since its introduction may be established, when pigs are killed on a holding following confirmation of an outbreak in accordance with Article 5(1)(a) of Directive 2001/89/EC, blood samples for serological tests must be taken at random from the pigs when they are killed. 2. The minimum number of pigs to be sampled must allow for the detection of 10% seroprevalence with 95% confidence in pigs in each subunit of the holding. Samples for virological tests may also be taken in accordance with the instructions of the competent authority, which will take into account the range of tests that can be performed, the sensitivity of the laboratory tests that will be used and the epidemiological situation. 3. However, in case of secondary outbreaks, the competent authority may decide to derogate from subparagraphs 1 and 2 and establish ad hoc sampling procedures, taking into account the epidemiological information already available on the source and means of virus introduction into the holding and the potential spread of disease from the holding.
3rd	To assess the effectiveness of disease‐specific sampling procedures based on clinical (ToR1.1) and laboratory (ToR1.2) examinations of the animals of listed species belonging to the categories described in article 13(2)) of an affected establishment, in order to grant a specific derogation from killing these animals, while ensuring that they do not pose a risk for the transmission of the disease.	No specific guidelines were found	No specific guidelines were found
5th	To assess the effectiveness of disease‐specific sampling procedures, based on clinical (ToR1.1) and laboratory (ToR1.2) examinations of the wild animals of listed species within the affected establishment and in its surroundings. The purpose of the sampling procedures is to ensure the detection of the virus, if the virus is present in these wild species.	No specific guidelines were found	No specific guidelines were found
6th	To assess the effectiveness of disease‐specific sampling procedures based on clinical (ToR1.1) and laboratory (ToR1.2) examinations of the animals of listed species in establishments located in the protection zone. The purpose of the sampling procedures is to ensure the detection of the virus, if the virus is present in these animals.	Article 10 of Directive 2001/89/EC: 1. Member States shall ensure that the following measures are applied in the protection zone: a) a census of all the holdings shall be made as soon as possible; after the establishment of the protection zone these holdings shall be visited by an official veterinarian within not more than seven days for a clinical examination of the pigs and for a check of the register and of the pig identification marks referred to in Articles 4 and 5 of Directive 92/102/EEC; e) all dead or diseased pigs on a holding shall be immediately notified to the competent authority, which shall carry out appropriate investigations in accordance with the procedures laid down in the diagnostic manual → 4. The measures in the protection zone shall continue to be applied at least until: a)… b) pigs on all holdings have undergone clinical and laboratory examinations carried out in accordance with the diagnostic manual in order to detect the possible presence of classical swine fever virus. The examinations referred to in point b) shall not take place before 30 days have elapsed after the completion of preliminary cleaning and disinfection measures on the infected holdings. Annex, CHAPTER IV.F.: 1. In order that the measures referred to in Article 10 of Directive 2001/89/EC may be lifted in a protection zone, in all holdings in the zone: — a clinical examination must be carried out in accordance with the procedures laid down in A.2 and 3; (2. When an official veterinarian visits a suspected holding to confirm or rule out classical swine fever: — a check of the production and health records of the holding must be carried out, if these records are available; — an inspection in each subunit of the holding must be carried out to select the pigs to be clinically examined. The clinical examination must include the taking of body temperature and must primarily concern the following pigs or group of pigs: — sick or anorexic pigs; — pigs recently recovered from disease; — pigs recently introduced from confirmed outbreaks or from other suspected sources; — pigs kept in subunits recently visited by external visitors which had a recent close contact with classical swine fever suspected or infected pigs or for which other particularly risky contacts with a potential source of classical swine fever virus have been identified; — pigs already sampled and serologically tested for classical swine fever, in case the results of these tests do notallow to rule out classical swine fever, and in‐contact pigs. 3. When reference is made to this paragraph, the clinical examination in the holding in question must be carried out on pigs selected at random in the subunits for which a risk of introduction of classical swine fever virus has been identified or is suspected. The minimum number of pigs to be examined must allow for the detection of fever if it occurs at a prevalence of 10% with 95% confidence in these subunits. However, in case of: — breeding sows, the minimum number of sows to be examined must allow for the detection of fever if it occurs at a prevalence of 5% with 95% confidence; — at semen collection centres, all boars must be examined.) Specific Domestic Animal Infectious Disease Quarantine Guidelines on Classical Swine Fever: Classical Swine Fever Diagnostics Manual (Ministry of Agriculture, Forestry and Fisheries of Japan, 2013): Test of surrounding farms in restriction areas: (1) Test to confirm the outbreak status When the outbreak of classical swine fever is confirmed, a prefecture shall enter farms (limited to those that rear six or more pigs, etc.) in areas of restricted movement and conduct the following tests within 24 h as a general rule: (i) Clinical test The prefecture shall enter farms (limited to those that rear six or more pigs, etc.) in areas of restricted movement to confirm the existence or absence of clinical signs set forth in Subsection 4, Item 1.	Annex, CHAPTER IV.F.: 2. The minimum number of blood samples to be taken must allow for the detection of 10% seroprevalence with 95% confidence in pigs in each subunit in the holding. However, in the case of: — breeding sows, the minimum number of samples to be taken must allow for the detection of 5% seroprevalence with 95% confidence; — a semen collection centre, blood samples must be taken from all boars. G. Specific Domestic Animal Infectious Disease Quarantine Guidelines on Classical Swine Fever: Classical Swine Fever Diagnostics Manual (Ministry of Agriculture, Forestry and Fisheries of Japan, 2013): Test of surrounding farms in restriction areas: (1) Test to confirm the outbreak status When the outbreak of classical swine fever is confirmed, a prefecture shall enter farms (limited to those that rear six or more pigs, etc.) in areas of restricted movement and conduct the following tests within 24 hours as a general rule: (ii) Blood test, antigen test and serum antibody test At the time of (i), blood test (leucocyte counting and confirming shift to the left of neutrophilic leukocyte nucleus), antigen test (PCR test, however, dead pigs, etc. shall be tested by the fluorescent antibody method, using amygdalae) and serum antibody test (ELISA method) shall be implemented to a given number of pigs. (2) Free Status Confirmation Test A similar test as (1) shall be conducted when 17 days have elapsed since the completion of quarantine measures at all infected farms in areas of restricted movement to confirm if the area is free from the disease. The number of collected samples for various tests in a test to confirm the outbreak status and a test to confirm disease‐free status is at least 30 (at least five randomly selected from each pig sty) as number enough to expose 10% infection at 95% reliability after consultation with the Animal Health Division and if there is more than one pigsty, samples shall be collected from all pigsties. In addition, Exhibit 1 ‘Classical Swine Fever Diagnostics Manual’ shall be referred to when conducting tests. Besides, samples shall be collected from abnormal pigs and if such pigs, etc. are not recognised, samples should be randomly collected from healthy pigs, etc.
8th	To assess the effectiveness of disease‐specific sampling procedures, based on clinical (ToR1.1) and laboratory (ToR1.2) examinations of the animals of listed species, for the sampling of the establishments located within the surveillance zone. The purpose of the sampling procedure is to ensure disease detection if the virus is present in establishments within the surveillance zone.	Article 11 of Directive 2001/89/EC: 1. Member States shall ensure that the following measures are applied in the surveillance zone: (a) a census shall be taken of all pig holdings; (e) all dead or diseased pigs on a holding shall be immediately notified to the competent authority, which shall carry out appropriate investigations in accordance with the procedures laid down in the diagnostic manual. → 3. The measures in the surveillance zone shall continue to be applied at least until: a)/…/ b) pigs on all holdings have undergone clinical and, where necessary, laboratory examinations as laid down in the diagnostic manual in order to detect the possible presence of classical swine fever virus. The examinations referred to in point b) shall not take place before 20 days have elapsed after the completion of preliminary cleaning and disinfection measures on the infected holdings.Annex, CHAPTER IV.G: 1. In order that the restrictions referred to in Article 11 of Directive 2001/89/EC may be lifted in a surveillance zone, a clinical examination must be carried out in all holdings in the zone in accordance with the procedures laid down in A.2.	Annex, CHAPTER IV.G 1./…/ In addition, blood samples for serological tests must be taken from pigs: — in all the holdings where no pigs of between two and eight months of age are kept; — whenever the competent authority deems that classical swine fever might have spread unnoticed amongst breeding sows; — in any other holding where sampling is deemed necessary by the competent authority; — in all semen collection centres 2. Whenever blood sampling for serological tests is carried out in holdings located in the surveillance zone, the number of blood samples to be taken in these holdings must be in accordance with F.2. However, if the competent authority deems that classical swine fever might have spread unnoticed amongst breeding sows, sampling may only be carried out in the subunits where these animals are kept.
Derogations to allow animal movements
9th	To assess the effectiveness of disease‐specific sampling procedures based on clinical and/or laboratory examinations of the animals of an establishment in a protection zone, in order to grant a derogation from prohibitions in the movement of animals, and allow for the animals to be moved to a slaughterhouse located within the protection zone or in the surveillance zone or outside the restricted zone (Art29).	Annex, CHAPTER IV.D.: 1. Without prejudice to the provisions of Article 11(1)(f), second subparagraph of Directive 2001/89/EC, in order that authorisation may be given to move pigs from holdings located in protection or surveillance zones in accordance with Article 10(3) of the said Directive, the clinical examination to be carried out by an official veterinarian must: — be carried out within the 24‐h period before moving the pigs; — be in accordance with the provisions laid down in A.2. 3. In case of pigs to be moved to a slaughterhouse, to a processing plant or to other places to be then killed or slaughtered, in addition to the investigations to be carried out in accordance with subparagraph 1, a clinical examination of pigs must be carried out in each subunit in which the pigs to be moved are kept. In case of pigs older than three to four months, this examination must include the taking of temperature of a proportion of pigs. The minimum number of the pigs to be checked must allow for the detection of fever if it occurs at a prevalence of 20% with 95% confidence in the subunits in question. However, in the case of breeding sows or boars, the minimum number of pigs to be examined must allow for the detection of fever if it occurs at a prevalence of 5% with 95% confidence in the subunit where the pigs to be moved are kept.	Annex, CHAPTER IV.D.: 4. When the pigs referred to in subparagraph 3 are slaughtered or killed, blood samples for serological tests or blood or tonsils samples for virological tests must be taken from pigs proceeding from each of the subunits from which pigs have been moved. The minimum number of samples to be taken must allow for the detection of 10% seroprevalence or virus prevalence with 95% confidence in each subunit. However, in the case of breeding sows or boars the minimum number of pigs to be sampled must allow for the detection of 5% of seroprevalence or virus prevalence with 95% confidence in the subunit where these pigs were kept. The type of samples to be taken and the test to be used will be in accordance with the instructions of the competent authority, which will take into account the range of tests that can be performed, the sensitivity of these tests and the epidemiological situation.
12th	To assess the effectiveness of disease‐specific sampling procedures based on clinical and/or laboratory examinations of the animals of an establishment in a protection zone, in order to grant derogation from prohibitions in the movement of these animals to a plant approved for processing or disposal of animal by‐products in which the kept animals are immediately killed (Art37).	See scenario 9	See scenario 9
13th	To assess the effectiveness of disease‐specific sampling procedures based on clinical and/or laboratory examinations of the animals of listed species in order to grant derogation from prohibitions and allow for these animals to be moved : a) from an establishment in a surveillance zone to a slaughterhouse located within or outside the restricted zone, b)from an establishment outside the surveillance zone to a slaughterhouse situated in the surveillance zone.	See scenario 9	See scenario 9
15th	To assess the effectiveness of disease‐specific sampling procedures based on clinical and/or laboratory examinations of kept ungulates of listed species in order to grant derogation and allow for them to be moved from an establishment in the surveillance zone to an establishment belonging to the same supply chain, located in or outside the surveillance zone, in order to complete the production cycle before slaughter.	Annex, CHAPTER IV.D.: 1. Without prejudice to the provisions of Article 11(1)(f), second subparagraph of Directive 2001/89/EC, in order that authorisation may be given to move pigs from holdings located in protection or surveillance zones in accordance with Article 10(3) of the said Directive, the clinical examination to be carried out by an official veterinarian must: – be carried out within the 24‐h period before moving the pigs; – be in accordance with the provisions laid down in A.2. 2. In the case of pigs to be moved to another holding, in addition to the investigations to be carried out in accordance with subparagraph 1, a clinical examination of pigs must be carried out in each subunit of the holding in which the pigs to be moved are kept. In case of pigs older than three to four months, this examination must include the taking of temperature of a proportion of pigs. The minimum number of pigs to be checked must allow for the detection of fever if it occurs at a prevalence of 10% with 95% confidence in these subunits. However, in the case of: – breeding sows, the minimum number of sows to be examined must allow for the detection of fever if it occurs at a prevalence of 5% with 95% confidence in the subunit where the sows to be moved are kept; – boars, all boars to be moved must be examined.	No specific guidelines were found
18th	To assess the effectiveness of disease‐specific sampling procedures based on clinical and/or laboratory examinations of the animals of an establishment located in the restricted zone of an outbreak in order to allow their move within the restricted zone, when restriction measures are maintained beyond the period set out in Annex XI.	Scenario 9 to slaughter and killing Article 10 of Directive 2001/89/EC: 2. Where the prohibitions provided for in paragraph 1 are maintained beyond 30 days because of further outbreaks of the disease and as a result animal welfare or other problems arise in keeping the pigs, subject to the conditions set out in paragraph 3, the competent authority may, following a reasoned application by the owner, authorise removal of pigs from a holding within the protection zone, to be directly transported to: a) a slaughterhouse designated by the competent authority, preferably within the protection or surveillance zone for the purpose of immediate slaughter; b) a processing plant or a suitable place where the pigs are immediately killed and their carcases are processed under official supervision; or c) under exceptional circumstances, to other premises located within the protection zone. Member States availing themselves of this provision shall immediately inform the Commission thereof in the Standing Veterinary Committee. 3. When reference is made to this paragraph, the competent authority may authorise removal of pigs from the holding concerned, on condition that: a) a clinical examination of the pigs in the holding and in particular those to be moved, including the taking of the body temperature of a proportion thereof, and a check of the register and the pig identification marks referred to in Articles 4 and 5 of Directive 92/102/EEC have been carried out by an official veterinarian; Article 11 of Directive 2001/89/EC: 2. Where the prohibitions provided for in paragraph 1 are maintained beyond 30 days because of further outbreaks of the disease and where as a result animal welfare or other problems arise in keeping the pigs, subject to the conditions set out in Article 10(3), the competent authority may, following a reasoned application by the owner, authorise removal of pigs from a holding within the surveillance zone to be directly transported to: a) a slaughterhouse designated by the competent authority, preferably within the protection or surveillance zone for the purpose of immediate slaughter; b) a processing plant or a suitable place where the pigs are immediately killed and their carcases are processed under official supervision; or c) under exceptional circumstances, to other premises located within the protection or surveillance zone. Member States availing themselves of this provision shall immediately inform the Commission thereof in the Standing Veterinary Committee.	Article 10 of Directive 2001/89/EC: 3. When reference is made to this paragraph, the competent authority may authorise removal of pigs from the holding concerned, on condition that: (e) if the pigs are to be slaughtered or killed, a sufficient number of samples shall be taken from the pigs in accordance with the diagnostic manual in order that the presence of classical swine fever virus in these holdings can be confirmed or ruled out; See scenario 9
Repopulation
19th	To assess the effectiveness of disease‐specific sampling procedures based on laboratory examinations of the animals that are kept for the repopulation prior to their introduction to rule out the presence of the disease.	No specific guidelines were found	Article 13 of Directive 2001/89/EC: 2. The reintroduction of pigs shall take account of the type of farming practised on the holding concerned and must conform to the following procedures: a) as regards open‐air pig holdings, the reintroduction of pigs shall start with the introduction of sentinel pigs which have been checked and found negative for the presence of antibodies against classical swine fever virus/…/
20th	To assess the effectiveness of disease‐specific sampling procedures based on laboratory examinations of the animals that have been repopulated, in the event of unusual mortalities or clinical signs being notified during the repopulation; to rule out the presence of the disease.	No specific guidelines were found	Annex, CHAPTER IV.E: 2. After re‐introduction of pigs, the competent authority shall ensure that in case of any disease or death of the pigs in the holding due to unknown reasons, the pigs in question are immediately tested for classical swine fever. These provisions shall apply until the restrictions referred to in Article 13(2)(a), second subparagraph and Article 19(8), second subparagraph (b), second sentence of Directive 2001/89/EC are lifted in the holding in question.
21st	To assess the effectiveness of disease‐specific sampling procedures based on laboratory examinations of the animals that have been repopulated, on the last day of the monitoring period calculated forward from the date on which the animals were placed in the repopulated establishment. In case the repopulation takes place in several days, the monitoring period will be calculated forward from the last day in which the last animal is introduced in the establishment.	No specific guidelines were found	Article 13 of Directive 2001/89/EC: 2. The reintroduction of pigs shall take account of the type of farming practised on the holding concerned and must conform to the following procedures: a) as regards open‐air pig holdings, the reintroduction of pigs shall start with the introduction of sentinel pigs which have been checked and found negative for the presence of antibodies against classical swine fever virus or come from holdings not subjected to any restrictions related to classical swine fever. The sentinel pigs shall be placed, in accordance with the requirements of the competent authority, throughout the infected holding and be sampled 40 days after having been placed on the holding, and tested for the presence of antibodies, in accordance with the diagnostic manual. b) as regards all other forms of rearing, the reintroduction of pigs shall either take place in accordance with the measures provided for – in point a) or shall be based on total repopulation, provided that: – all the pigs arrive within a period of 20 days and come from holdings not subjected to any restrictions related to classical swine fever, – pigs in the repopulated herd are subjected to a serological examination in accordance with the diagnostic manual. Sampling for that examination shall be carried out at the earliest 40 days after the arrival of the last pigs, Annex, CHAPTER IV.E 1. When pigs are re‐introduced into a holding in accordance with Article 13(2)(a) or (2)(b) or Article 19(8), second subparagraph (b) of Directive 2001/89/EC, the following sampling procedures must be applied: — in case sentinel pigs are reintroduced, blood samples for serological tests must be taken at random from a number of pigs that allow for the detection of 10% seroprevalence with 95% confidence in each subunit of the holding; — in case of total re‐population, blood samples for serological tests must be taken at random from a number of pigs that allow for the detection of 20% seroprevalence with 95% confidence in each subunit of the holding. However, in the case of breeding sows or boars the number of samples to be taken must be such as to detect 10% seroprevalence with 95% confidence.