Table 4.
Effect of PGRs in shoot regeneration medium (SRM) on protoplast regeneration of rapeseed.
| PGR conc. (mg l−1) | Regeneration (%)* | PGR conc.(mg l−1) | Regeneration (%) |
|---|---|---|---|
| BAP 2.0NAA 0.1 | 0.0 c | Kinetin 2.0NAA 0.1 | 0.0 c |
| BAP 3.0NAA 0.2 | 0.0 c | TDZ 0.5NAA 0.1 | 0.0 c |
| BAP 5.0NAA 0.5 | 1.0 c | TDZ 1.1NAA 0.1 | 5.0 c |
| Zeatin 1.0NAA 0.1 | 0.0 c | TDZ 2.2NAA 0.5 | 45.0 a |
| Zeatin 2.0NAA 0.2 | 0.0 c | TDZ 2.2NAA 1.0 | 22.0 b |
SRM medium composition: Full strength MS, sucrose 20 g l−1, 0.5 mg l−1 AgNO3, 2.5 g l−1 Gelrite at pH 5.7.
*
The results were recorded after one month on the SRM medium. Values followed by the same letter were not statistically different at p = 0.05 (n = 3).