Table 5.
Effect of C-sources in shoot regeneration medium (SRM) on protoplast regeneration of rapeseed.
Sugar conc. (g l−1) | Regeneration (%)* | Sugar conc. (g l−1) | Regeneration (%) |
---|---|---|---|
Sucrose 15 | 30.6 b | Glucose 10 | 11.3 c |
Sucrose 20 | 41.0 a | Glucose 20 | 10.0 c |
Sucrose 30 | 31.4 ab |
SRM composition: Full strength MS, 2.2 mg l−1 TDZ, 0.5 mg l−1 NAA, 0.5 mg l−1 AgNO3, 2.5 g l−1 Gelrite at pH 5.7.
The results were recorded after one month on the SRM medium. Values followed by the same letter were not statistically different at p = 0.05 (n = 3).