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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Cancer Res. 2020 Apr 7;80(11):2125–2137. doi: 10.1158/0008-5472.CAN-19-3018

Figure 1. PADI4 is expressed in human breast cancer cell lines.

Figure 1.

(A) PADI4 mRNA expression normalized to GAPDH in human breast cancer cell lines as assessed by RT-qPCR. t-test for difference between basal and luminal subclasses. (B) PADI1-4 mRNA expression in representative breast cancer cell lines as assessed by RT-qPCR. Dunnett’s multiple comparison test with PADI4 as comparator. (C-D) Western blots of nuclear PADI4 protein expression in luminal (panel C) and basal A and basal B (panel D) breast cancer cell lines. Lamin B1, loading control. (E-F) Western blots of nuclear PADI4 following shRNA knockdown in MCF10Ca1h, MCF7E cells (E) and MDA-MB-231 LM2 cells (F) cells. (G) RT-qPCR of PADI4 following knockdown (mean +/−SD, n=3; t-test). (H) ELISA for total citrullinated histone H3. Results are normalized to shCON (mean +/− SD, n=3; Dunnett’s multiple comparison test with shCON as the comparator. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.