Skip to main content
. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Cancer Res. 2020 Apr 7;80(11):2125–2137. doi: 10.1158/0008-5472.CAN-19-3018

Figure 3. PADI4 inhibition has widespread effects on Histone H3 distribution but little effect on the transcriptome.

Figure 3.

(A-D) Heatmaps for the difference in ChIP-seq signal in GSK484 (PADI4 inhibitor) vs GSK106 (control) treated MCF10Ca1h cells for H3 (A), H3R2/8/17citr (B), H3K27me3 (C) and H3K4me3 (D) at the transcriptional start site (TSS) +/− 10kb regions for all Ref-Seq NM transcripts. The heatmaps are sorted by H3. GSK484 and show ChIP-seq intensity (log2(ChIP/IgG)). Treatment duration was 3 days. (E) Corresponding heatmap for the difference in RNA-seq data (GSK484 vs CON). (F-H) Heatmaps for the difference in H3 ChIP-seq signal at promoters (F: +/− 4kb of TSS); proximal enhancers (G: enhancer elements located within 4kb of the TSS); and distal enhancers (H: enhancers located >4 kb distal to a TSS). S and E show start and end of enhancer element. The graphs above each heatmap show the average signal intensity across the region for all loci.