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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Cancer Res. 2020 Apr 7;80(11):2125–2137. doi: 10.1158/0008-5472.CAN-19-3018

Figure 5. The PADI4-regulated transcriptome is associated with outcome in clinical breast cancer datasets.

Figure 5.

(A) Schematic for identification of PADI4-regulated transcripts in MCF10Ca1h cells following long-term inhibition of PADI4 by genetic knockdown. (B-C) Ingenuity Pathway analysis ) of top upregulated diseases and functions (B), and upstream regulators (C) derived from core analysis of the 181 differentially expressed genes (DEGs) reflecting PADI4 inhibition. (D) Hallmark GSEA analysis identifies “Epithelial Mesenchymal Transition” gene set as significantly enriched. NES, normalized enrichment score. (E) Kaplan Meier overall survival curves for breast cancer patients in METABRIC dataset, stratified by higher than median (red) or lower than median (blue) expression of a PADI4 inhibition signature. n=number of patients/group. ER, estrogen receptor. For details of signature generation, see Supplementary Methods.