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. Author manuscript; available in PMC: 2021 Jul 21.
Published in final edited form as: Cancer Res. 2020 Apr 7;80(11):2125–2137. doi: 10.1158/0008-5472.CAN-19-3018

Table 1. Effect of PADI4 knockdown on tumor-initiating ability of breast cancer cell lines in vivo.

Cells were orthotopically implanted in nude mice at the specified cell inocula, and tumor formation was assessed at day 60. Cancer stem cell frequency was calculated from tumor incidence rates using ELDA software.

Tumor incidence at different cell inocula
Model Cell inoculum 2.5×10^5 5×10^4 1×10^4 1×10^3 1/stem cell frequency Confidence interval Relative stem cell frequency Chi2 p-value
MCF10Ca1h Parent 10/10 5/10 0/10 0/10 75410 139349-40809 1
shCON 10/10 8/10 0/10 0/10 45474 85992-24047 1.65 0.29
shPADI4 79 10/10 10/10 3/10 0/10 18760 18760-10053 4.02 0.00368
shPADI4 80 10/10 10/10 3/10 0/10 17575 17575-9217 4.29 0.00263
Cell inoculum 2.5×10^5 1×10^5 1×10^4 1×10^3 1/stem cell frequency Confidence interval Relative stem cell frequency Chi2 p-value
MCF10Ca1a Parent ND 8/10 3/10 0/10 52177 103175-26387 1
shCON ND 8/10 5/10 0/10 41253 82167-20712 1.26 0.61
shPADI4 79 ND 8/10 5/10 0/10 8469 17493-4101 6.16 0.000511
shPADI4 80 ND 10/10 7/10 1/10 7366 14958-3627 7.08 0.000128
Cell inoculum 2.5×10^5 1×10^5 1×10^4 1×10^3 1/stem cell frequency Confidence interval Relative stem cell frequency Chi2 p-value
MCF7E Parent ND 8/10 2/10 0/10 59215 117500-29842 1
shCON ND 8/10 1/10 0/10 67767 135682-33846 0.87 0.787
shPADI4 79 ND 10/10 3/10 0/10 25058 53620-11710 2.36 0.101
shPADI4 80 ND 10/10 4/10 2/10 20275 44941-9148 2.92 0.0435
Cell inoculum 2.5×10^5 2×10^5 2×10^4 2×10^3 1/stem cell frequency Confidence interval Relative stem cell frequency Chi2 p-value
MDA-MB-231 LM2 shCON ND 10/10 7/10 1/10 16938 34985-8201 1
shPADI4 80 ND 10/10 10/10 6/10 2180 4957-959 7.77 0.000265