Fig. 7.

Microglia in whole brain 2 months after experimental NEC. Rat pups were subjected to experimental NEC and surviving pups were placed with surrogate dams. Rats were sacrificed at two months of age, and brains harvested and fixed in 4% PFA. Frozen sections were stained with Iba-1 antibody and Alexa 488 conjugated secondary antibody. At least 8 confocal pictures per section were imaged using 20X objectives. (A) Representative immunohistochemical images of the whole brain; (B) percent of Iba-1+ cells/HPF quantified using Image J software; (C) numbers of de-ramified/ameboid microglia manually counted/HPF. Error bars represent SEM with statistical significance defined as p ​≤ ​0.05 (one-way ANOVA).