Fig. 9.

Myelin Basic Protein (MBP) in hippocampus 2 months after experimental NEC. Rat pups were subjected to experimental NEC and surviving pups were placed with surrogate dams. Rats were sacrificed at two months of age, brains were harvested, and hippocampus sections were fixed in 4% PFA. Frozen sections were stained with MBP antibody and Alexa 488 conjugated secondary antibody. At least 5 confocal pictures per section were imaged using 20X objectives. (A) Representative immunohistochemical images from hippocampus sections; (B) densitometry analysis using Zen software; (C) % positive MBP staining/HPF analyzed using Image J software. Error bars represent SEM with statistical significance defined as p ​≤ ​0.05 (one-way ANOVA). (D) Selective images were shown in 63X objectives.