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. Author manuscript; available in PMC: 2022 Jun 28.
Published in final edited form as: Analyst. 2021 Jun 28;146(13):4340–4347. doi: 10.1039/d1an00399b

Figure 1. Padlock Rolling Circle Amplification Nuclease Protection Lateral Flow Assay (PLAN-LFA) Schematic.

Figure 1

Padlock probe hybridization with target DNA oligo enables sequence specific ligation of the 5’ and 3’ end of the padlock probe by T4 DNA ligase. The target-primed circular template undergoes rolling circle amplification in the presence of Phi29 DNA polymerase. The resulting ssDNA product binds the labeled nuclease protection probe. Upon P1 nuclease digestion, the protected labeled NPA probes are detected in an immunoassay format.