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A. Gel electrophoresis data showing large molecular weight RCA product is generated in the presence of all assay components (T) which remains in the well of the gel. No product is observed in the absence of the padlock probe (C1) or Target oligo (C2) or T4 DNA ligase (C3) or Phi29 DNA polymerase (C4). B. P1 nuclease digested and undigested samples were run on lateral flow strips (n =3).
