Figure 5. RNA detection using PLAN-LFA:

A. Dose-response with purified SARS-CoV-2 RNA. Using blank + 3.3$\sigma$ a limit of detection of approximately 1.78×10⁵ copies/mL was obtained. B. PLAN-LFA performed on bacterial cultures of E. coli transformed with ampicillin resistance (test condition) and chloramphenicol resistance (negative control). ssDNA oligo target positive (10 pM) and negative samples were used to control for assay performance. The ssDNA oligo spiked in chloramphenicol resistant culture was used to control for sample matrix effects.