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Off-target, collagen content assessment. A) Kidney and liver sections from PBS-, MCG PAM-, and SCG PAM-treated mice stained with picrosirius red show collagen (red) content. Scale bars 100 μm. B) Quantification of the collagen content demonstrate there are no significant differences or enhancements of collagen among MCG PAM- or SCG PAM-treated mice, indicating a lack of off-target effects from the nanoparticles (N = 4 mice for MCG PAM and SCG PAM and N = 3 mice for PBS).
