Fig. 1.
Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown