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. 2020 Dec 21;22(4):bbaa337. doi: 10.1093/bib/bbaa337

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Results provided by the FireProtASR workflow using haloalkane dehalogenase DhaA as an input query. (A) Phylogenetic tree of the HLD-II constructed by the FireProtASR strategy with indicated three global ancestors reconstructed within this study. (B) Phylogenetic tree for the local ancestor of the ancestral node 172. (C) Phylogenetic tree for the local ancestor of ancestral node 230. (D) Phylogenetic tree for the local ancestor of ancestral node 238. (E) Multiple sequence alignment comparing the query sequence with the suggested ancestral sequences and the result of the back-to-consensus analysis.