. 2021 Jul 21;16(7):e0253754. doi: 10.1371/journal.pone.0253754

Table 3. Effect of biochanin A on rumen fluid pH during a 40% and 70% corn SARA challenge at 0 (pre-feeding), 2, 4, and 8 h (true means; n = 3).

Hour¹	SARA Challenge Period²	Treatments³				Statistics⁴
Hour¹	SARA Challenge Period²	HF CON	SARA CON	MON CON	BCA	Sig	SEM
0	60:40	6.64^a	6.16^cα	6.39^bα	6.48^bα	P < 0.01	0.03
0	30:70	6.65^a	5.58^cβ	5.99^bβ	5.94^bβ	P < 0.01	0.03
2	60:40	6.02^a	5.56^dα	5.71^cα	5.83^bα	P = 0.01	0.03
2	30:70	6.09^a	5.36^cβ	5.61^bβ	5.68^bβ	P = 0.01	0.03
4	60:40	6.12^a	5.49^dα	5.76^cα	5.92^bα	P < 0.01	0.02
4	30:70	6.10^a	5.28^dβ	5.43^cβ	5.59^bβ	P < 0.01	0.02
8	60:40	6.22^a	5.72^dα	5.82^cα	6.00^bα	P < 0.01	0.03
8	30:70	6.23^a	5.28^dβ	5.65^cβ	5.79^bβ	P < 0.01	0.03

¹Hour 0 is pre-feeding. Hours 2, 4 and 8 are post-feeding.

²60:40 → 60% basal diet:40% cracked corn; 30:70 → 30% basal diet:70% cracked corn.

³HF CON: High Fiber Control (basal diet only; corn silage + dried distillers’ grains, to meet protein requirements); SARA CON: SARA Control; MON CON: SARA + Monensin (200 mg d^-1); BCA: SARA + Biochanin A (6 g d^-1).

⁴Values with different English letters (^a,b,c,d) are statistically different within period between treatments (P < 0.05); Values with different Greek letters (^α,β) are statistically different within treatment between periods (P < 0.05).