Table 2. Quantification of GFP localization for tagged diguanylate cyclases.
Cells with indicated number of foci (%) | |||||||
---|---|---|---|---|---|---|---|
DGC | total cells (n) | diffuse/no foci | 1 | 2 | 3 | 4 | 5 |
2884 | 55 | 0 | 42 | 33 | 16 | 9 | 0 |
2884ΔTM | 101 | 55 | 20 | 13 | 2 | 0 | 11 |
Images from Fig 3 were analyzed with Fiji ImageJ software to assess localization of indicated proteins. Foci were counted per cell and populations consisting of different localization patterns were placed into the indicated categories and population percentages were reported.