Figure 7.

Modulation of interstitial macrophage populations in the absence of CX3CR1 signaling during fungal-associated allergic airway inflammation. C57BL/6 [wild-type (WT)] and CX3CR1-deficient (Cx3cr1-/-) mice were chronically exposed to Aspergillus fumigatus as described in materials and methods. Twenty-four hours after the last organism challenge, lung cells were isolated by bronchoalveolar lavage, enumerated, Fc-blocked, stained with a live/dead staining kit, and stained for interstitial macrophage population 1 (IM1; CD11c lo MHCII lo; A), interstitial macrophage population 2 (IM2; CD11c lo MHCII hi; B), and interstitial macrophage population 3 (IM3; CD11c hi MHCII hi; C). A–C illustrate cumulative data from 3 independent studies (n = 4–5 mice per group per study). *P < 0.05 and **P < 0.01, respectively (two-tailed Student’s t test).