. Author manuscript; available in PMC: 2021 Jul 21.

Published in final edited form as: ACS Catal. 2020 Jun 5;10(12):6969–6974. doi: 10.1021/acscatal.0c01892

Table 1.

Apparent Kinetic Parameters for Substrates

		4-HBA			3,4-DHBA
	variant	K_M^a (μM)	k_cat^a (s⁻¹)	k_cat/K_M^a(μM⁻¹ s⁻¹)	K_M^a (μM)	k_cat^a (s⁻¹)	k_cat/K_M^a(μM⁻¹ s⁻¹)
this study	wild-type	48 ± 7	7.6 ± 0.1	0.16 ± 0.03	−	−	−
	DA015^b	24 ± 10	1.9 ± 0.2	0.079 ± 0.04	48 ± 10	3.0 ± 0.2	0.062 ± 0.01
	DA016^c	120 ± 10	4.0 ± 0.1	0.034 ± 0.001	120 ± 10	4.1 ± 0.2	0.033 ± 0.004
Chen et al.¹⁷	Y385F/T294A	48 ± 3	0.90 ± 0.03	0.019 ± 0.001	160 ± 30	1.7 ± 0.2	0.012 ± 0.003
Moriwaki et al.⁶	Y385F/L199 V	22 ± 5	0.45 ± 0.02	0.021 ± 0.004	52 ± 16	4.4 ± 0.4	0.085 ± 0.02
	wild-type	39 ± 6	8.9 ± 0.5	0.23 ± 0.03	42 ± 17	0.33 ± 0.04	0.0078 ± 0.002

Apparent kinetic parameters shown for constant NADPH concentration of 1 mM. Data by Chen et al.¹⁷ and Moriwaki et al.⁶ is presented as it appeared in the references.

DA015, L199R-T294C-Y385M.

DA016, V47I-L199N-T294A-Y385I.