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. 2021 Jul 12;10:e60810. doi: 10.7554/eLife.60810

Figure 2. Two distinct morphologies of Lpar1-EGFP SPNs in S1BF.

Figure 2.

(a) Streptavidin labeled morphologies of 2 EGFP+ SPNs recorded at P3; arrowhead, pyramidal subtypes with apical dendrite projecting at ~45° into L6a; arrow, fusiform morphology with horizontal, bitufted dendrites largely restricted to the SP (scale bar = 25 μm). (b) Reconstructed (ImageJ) dendritic arbors of the cells shown in (a) were used to calculate directionality with 90° indicative of vertically orientated dendrites and ~0° primarily horizontal dendrites (scale bar = 25 μm). (c) A difference was observed in the dendritic orientation of fusiform and pyramidal SPNs (Mann–Whitney U = 5, fusiform n = 7, median = 4.38; pyramidal n = 12, median = 54.89, **p<0.001 two-tailed). Overlay of axonal arbors of (d) pyramidal (recovered between P1 and P8) and (e) fusiform (recovered P1and P5) cells aligned on soma location (horizontal position indicated by the blue circle); approximate barrel location indicated by grey-shaded areas for P5+ cells; scale bar = 180 μm. (f) Proportion of fusiform (grey) and pyramidal (black) Lpar1-EGFP SPNs over the first postnatal week. (g) Immunohistochemistry for EGFP (left) and GABA (middle); EGFP+/GABA+ cells indicated by the white arrows; right panel, overlay of EGFP (green) and GABA (red). (h) Schematic of the two morphological subtypes of Lpar1-EGFP SPN: pyramidal (Pyr) and fusiform SPNs encountered prior to P5; a putative synaptic connection between the two subtypes is indicated by the question mark. MZ, marginal zone; CP, cortical plate; SP, suplate.