Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, male) | Tg(Lpar1-EGFP)GX193Gsat | GENSAT Project at Rockefeller University | MGI:4847204; transgene insertion GX193 | Y Chromo- some linked |
| Strain, strain background (M. musculus, mixed sex) | Ssttm2.1(cre)Zjh/J | The Jackson Laboratory | Stock No: 013044; RRID:IMSR_JAX:013044 |
Maintained in Butt lab on C57BL/6 background |
| Strain, strain background (M. musculus, mixed sex) | Olig3tm1(cre)Ynka | Prof. Yasushi Nakagawa University Minnesota |
||
| Strain, strain background (M. musculus, mixed sex) | Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze /J | The Jackson Laboratory | Stock No: 012569; RRID:IMSR_JAX:012569 | Maintained in Butt lab on C57BL/6 background |
| Strain, strain background (M. musculus, mixed sex) | R26::P2x2r-EGFP (floxed-stop-rat P2x2 receptor) | Prof. Gero Miesenböck (Oxford) | Maintained in Butt lab on C57BL/6 background | |
| Antibody | Chicken anti-GFP (polyclonal) |
Abcam | ab13970 RRID:AB_300798 |
(1:250) |
| Antibody | Rabbit anti-GABA (polyclonal) | Sigma-Aldrich | Cat# A2052, RRID:AB_477652 | (1:1000) |
| Antibody | Guinea-pig anti-GABA (polyclonal) | Abcam | ab17413 RRID:AB_443865 |
(1:1500) |
| Antibody | Rabbit anti-Caspase three antibody, active (cleaved) form (polyclonal) | Merck Millipore | AB3623 RRID:AB_91556 |
(1:200) |
| Antibody | Goat anti-Chicken, Alexa Fluor 488 (polyclonal) | Abcam | ab150169 RRID:AB_2636803 |
(1:1000) |
| Antibody | Goat anti-Rabbit, Alexa Fluor 546 (polyclonal) | Thermo-Fisher | A11035 RRID:AB_143051 |
(1:1000) |
| Commercial assay or kit | In Situ Cell Death Detection Kit, TMR red | Roche, Sigma-Aldrich |
SKU: 12156792910; Lot #: 45197500 |
|
| Chemical compound, drug | NaCl; KCl; NaHCO3; NaH2PO4; MgCl2; CaCl2; K-gluconate; Li-GTP; Mg-ATP; HEPES; Gluconic acid; EGTA; SrCl2 |
Sigma-Aldrich | S7653 P4504 S6014 S3139 M2670 22,350–6 G4500 G5884 A9187 H3375 G1951 E4378 204463 |
|
| Chemical compound, drug | MNI-caged glutamate | Tocris Bioscience UK | Cat. No.: 1490. Lot no.: 48A/206152; 50A/255070; 50A/220558; 50A225070; 51A/240237; 51A/243753; 52A/249897; 52A/251627 |
100 µM |
| Chemical compound, drug | DMNPE-caged ATP | Life Technologies UK | A1049 | 100 µM |
| Chemical compound, drug | Biocytin | Sigma- Aldrich |
B4261; Lot no. SLCB0219; SLBS5344 |
|
| Chemical compound, drug | Streptavidin, Alexa Fluor 568 conjugate | Thermo-Fisher |
S11226 RRID:AB_2315774 |
(1:500) |
| Software, algorithm | GraphPad Prism Software | GraphPad Software | RRID:CR_002798 | |
| Software, algorithm | ImageJ/Fiji | Fiji | RRID:SCR_002285 | |
| Software, algorithm | pClamp 10 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | |
| Other | DAPI stain | Thermo-Fisher | D1306 RRID:AB_2629482 |
(1:1000) |