Reagent		Stock concetration			Final concentration		Volume (µl)
H2O							to 25 µl
HF Buffer		5×			1×		5
dNTPs		10 mM			200 µM		0.5
F Index		5 µM			100 nM		0.5
R Index		5 µM			100 nM		0.5
Polymerase		2000 U/µl			20 U/µl		0.25
template							2–5
Total							25