Figure 1.

CRTH2 deficiency results in enhanced worm expulsion from the small intestine.(a) Littermate or cohoused C57BL/6 WT and Gpr44^−/− mice were infected with 500 N. brasiliensis (Nb) L3 larvae subcutaneously, and on day 5 and day 7 p.i., intestinal worm burden was quantified. (b–d) On day 7 p.i., the percentage and total number of ILC2s (live, CD45⁺Lin⁻Klrg1⁺IL-17RB⁺Gata3⁺; b), eosinophils (live, CD45⁺Siglec F⁺CD11b⁺; c), and CD4⁺ Th2 cells (live, CD45⁺Lin⁺CD4⁺Gata3⁺; d) in the MLNs were assessed using flow cytometry. (e and f) On day 7 p.i., levels of IL-4 and IL-13 in the cell-free supernatant of MLN cells stimulated for 48 h with anti-CD3/anti-CD28 (3/28) were measured by ELISA (e), and the expression of Il4 and Il13 in small intestinal (SI) homogenates was measured by real-time PCR (relative to Actβ and normalized to WT naive [N]; f). Data are mean ± SEM. (a–f) Analyzed using a linear mixed-effects model with pairwise comparison. (a) Day 5 p.i., n = 4–6; two independent experiments. Day 7 p.i., n = 10 or 11; four independent experiments. (b) N, n = 3 or 4; Nb, n = 5 or 6; two independent experiments. (c) N, n = 7–9; Nb, n = 12–14; six independent experiments. (d) N, n = 5 or 6; Nb, n = 8–12; three independent experiments. (e) IL-4, N, n = 7; Nb, n = 8–11; four independent experiments. IL-13, N, n = 5 or 6; Nb, n = 6–8; three independent experiments. (f) Il4 N, n = 5–7; Nb, n = 7; three independent experiments. Il13 N, n = 6; Nb, n = 10–13; four independent experiments. Each n refers to number of mice/group in total across all experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.