Figure S1.

ILC2, eosinophil, Th2 cell, and small intestinal Type 2 cytokine responses in CRTH2-deficient mice. C57BL/6 WT and Gpr44^−/− mice were infected with 500 N. brasiliensis (Nb) L3 larvae subcutaneously. (a and b) ILC2s in the MLN were gated as live, CD45⁺Lin(CD3/CD5/TCRγδ/CD11b/CD11c/CD19/NK1.1)⁻CD127⁺Klrg1⁺Gata3⁺IL-17RB⁺ (a) or live, CD45⁺Lin(CD3/CD5/CD11b/CD11c/CD19/NK1.1)⁻CD127⁺CD90⁺ST2⁺CD4⁻ (b). (c) On day 7 p.i., the percentage and total number of ILC2s (live, CD45⁺Lin⁻CD127⁺CD90⁺CD25⁺ST2⁺CD4⁻) in the MLN were assessed using flow cytometry. (d) Eosinophils in MLN were gated as live, CD45⁺CD11b⁺Siglec F⁺. (e) CD4⁺ Th2 cells in the MLN were gated as live, CD45⁺Lin(CD3/CD5/CD11b/CD11c/CD19/NK1.1)⁺CD4⁺Gata3⁺. (f and g) On day 5 p.i., representative images showing immunofluorescent staining for Klrg1 in histological sections of the small intestine (SI; f) were quantified for the number of Klrg1⁺ cell per crypt/villus unit (g). Scale bar = 100 µm. (h) On day 7 p.i., IL-4 and IL-13 in small intestinal homogenates were measured by ELISA. Data are mean ± SEM. (a, b, d, and e) Representative plots. (c, g, and h) Analyzed using a linear mixed-effects model with pairwise comparison. (c) Naive (N), n = 7; Nb, n = 11–13; four independent experiments. (f and g) N, n = 5 or 6; Nb, n = 5 or 6 (at least 10 crypt/villus units were examined/mouse, and those values were averaged); two independent experiments. (h) IL-4 N, n = 5–9; Nb, n = 14; five independent experiments. IL-13 N, n = 6 or 7; Nb, n = 12–14; five independent experiments. Each n refers to number of mice/group in total across all experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. SSC-A, side scatter A.