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Spheroid DPSCs expressed a high level of pluripotent markers. Monolayer cultured cells (2D) showed significantly decreased pluripotent gene (OCT4, SOX2, and NANOG) expression as compared to 24-hour 3D cultured (3D 24 h), 48-hour 3D cultured (3D 48 h), and 72-hour 3D cultured (3D 72 h) cells. Asterisk (∗) represents a significant (p < 0.05) difference.
