Table 2.
Lists of primers used in RT-qPCR.
| Gene name | Primer sequence | Product size (bp) | Anneal. temp (°C) | Reference |
|---|---|---|---|---|
| OCT4 | F: ACTATCATTGATGCCCCAGGAC R: ACACCAGCAGCAACAATCAG |
128 | 60 | NM_021130.3 |
| SOX2 | F: CACCCACAGCAAATGACAGC R: GTCCCCCAAAAAGAAGTCCAG |
120 | 60 | NM_021130.3 |
| NANOG | F: TGCAACCTGAAGACGTGTG R: TGCAACCTGAAGACGTGTG |
153 | 60 | NM_024865.2 |
| FABP4 | F: TGAGATTTCCTTCATACTGG R: TGGTTGATTTTCCATCCCAT |
128 | 60 | NM_001442.2 |
| LPL | F: AGACACAGCTGAGGACACTT R: GCACCCAACTCTCATACATT |
137 | 60 | NM_001442.2 |
| PPARγ | F: TTGCTGTCATTATTCTCAGT R: GAGGACTCAGGGTGGTTCAG |
124 | 60 | AB565476.1 |
| Runx2 | F: CCTTGGGAAAAATTCAAGCA R: AACACATGACCCAGTGCAAA |
181 | 60 | NM_001015051 |
| OPN (osteopontin) | F: CATCACCTCACACATGGAAAGC R: CTGACTCGTTTCATAACTGTCC |
115 | 60 | NM_001251830.1 |
| ON (osteonectin) | F: GTGCAGAGGAAACCGAAGAG R: AAGTGGCAGGAAGAGTCGAA |
170 | 60 | J03040.1 |
| YWHAZ | F: ACGAAGCTGAAGCAGGAGAAG R: TTTGTGGGACAGCATGGATG |
111 | 60 | BC108281.1 |