Figure 4.

Effects of tetrahydrothieno[2,3-c]pyridine 3b and PNA-a221 on cell growth and viability. U251 cells were treated with the indicated concentrations of compound 3b and R8-PNA-a221 and (A) cell growth and cytotoxicity were determined. The anti-proliferative effects were assayed by employing a cell counter; cytotoxicity was evaluated by MTT assay. (B) Representative image of the clonogenic assay performed on U251 cells, and a summary of three independent experiments. Cells were fixed and stained with crystal violet after 10 days of treatment. (C) Morphological alteration of the treated U251 cells. Cells were treated as indicated and the morphological phenotype of the cells was analyzed by microphotographic analysis [magnification, ×100; combined treatment magnification, ×200 (left panel) and ×400 (right panel)]. PNA, peptide nucleic acid. ^*P<0.05, ^**P<0.01, vs. untreated cells.