Figure 5. Low dose sulindac does not affect PGE2 at the systematic level.

Plasma samples collected from mice were used to measure PGE2 levels using an ELISA assay. The same plasma sample sets for Figure 4 were utilized in this analysis. Plasma samples (50 μL) were appropriately diluted and added to a 96 well polystyrene microplate coated with goat anti-mouse polyclonal antibodies. After incubation with 50 μL of mouse PGE2 monoclonal antibody for 1 h at room temperature, the PGE2 conjugate was added to each well and incubated for 2 h with gentle shaking. After washing, the substrate solution was added to each well and incubated for up to 30 min, and then the stopping buffer was added to stop the color development reaction. Finally, the absorbance was measured at 450 nm using a microplate reader (Bio-Rad).