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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2021 Jun 2;45(7):1348–1358. doi: 10.1111/acer.14630

Figure 4.

Figure 4

Selectivity of two-hour incubation with siRNA targeting Kcnk12 and Kcnk13.

Comparison was made in the effect of incubation of coronal brain slices of the VTA (400 μm thick) with siRNA targeting Kcnk12, Kcnk13 or scrambled siRNA (siScr).

A. siKcnk13 or siScr incubation: Incubation with siKcnk13 significantly reduced the level of Kcnk13 (n=12) compared to siScr (n=14) and to Kcnk12 (n=13) Kcnk13 levels (one-way ANOVA, F2,36=7.77, P<0.01; means comparison with Bonferroni test, * = P<0.05 for significance).

B. siKcnk12 or siScr incubation: Incubation with siKcnk12 significantly reduced the level of Kcnk12 (n=12) compared to Kcnk13 (n=13) but not to siScr; there was no significant difference between Kcnk13 and Scr groups (one-way ANOVA, F2,34=3.28, * = P<0.05; means comparison with Bonferroni test, P<0.05 for significance).