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Intracellular S. Typhimurium infection in RAW 264.7 cells treated with fDNA and ATC/fDNA polyplexes at varying concentrations. RAW 264.7 cells were incubated with S. Typhimurium SL1344 for 1 h prior to overnight treatment with fDNA or polyplexes. Intracellular loading of S. typhimurium in RAW 264.7 cells was quantified by flow cytometry and visualized in corresponding fluorescence micrographs. Representative bright field and fluorescence micrographs are shown.
