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. 2021 Jul 21;11:14917. doi: 10.1038/s41598-021-94364-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HEK-293T cells expressing hAd5 S-Fusion + ETSD show enhanced spike receptor binding domain (S RBD)-specific antibody and recombinant ACE2 (rACE2) binding. Flow cytometric analysis of anti-S RBD antibody binding to construct-infected cells reveals surface expression of S RBD is very low in (a) S-WT or (b) S-WT + N-ETSD infected cells and is higher in (c) S-Fusion infected cells. The highest S RBD cell surface expression was seen for (d) S-Fusion + N-ETSD infected cells. rACE2 showed little binding to HEK-293T cells transfected with (e) S-WT, higher binding with (f) S-Fusion, and the highest binding with (g) S-Fusion + N-ETSD. Y-axis scale is normalized to mode (NM).