Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 21;12:4442. doi: 10.1038/s41467-021-24743-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Representative genome browser view of neonatal and adult H2Bub1 ChIP-seq replicates. Dashed red box highlights a gene with dynamic H2Bub1 occupancy. b Profile plot showing average H2Bub1 signal at gene bodies. Plot shows gene bodies and 50 kb upstream of the TSS or downstream of the TSE. c Genes with detectable H2Bub1 ChIP-seq signal at P1, P28, or both timepoints. d Distribution of changes in H2Bub1 signal intensity during maturation, for all genes marked by H2Bub1 in at least one timepoint. e H2Bub1 P1 ChIP signal (top) or the change of H2Bub1 ChIP signal during maturation (bottom) as a function of the gene’s maturational change in RNA expression. The change in RNA was binned to contain the indicated range of log2 fold-change values. The change in gene expression correlated with the change in H2Bub1 occupancy, but not with the absolute H2Bub1 occupancy. Numbers at the top of plot (n) indicate genes per bin. Center lines in box plots indicate the median, while boxes show 25th and 75th percentiles. Whiskers denote the maximum observation within the 75th percentile +1.5 times the interquartile range, or the minimum observation within the 25th percentile −1.5 times the interquartile range. P-value: Steel–Dwass vs. H2Bub1 P1 ChIP signal in the (−1,0] bin; ***P < 0.001. f Maturational change in expression versus maturational change in H2Bub1 signal plotted for genes differentially expressed in RNF20/40-depleted CMs. Color indicates the direction of differential expression in RNF20/40-depleted CMs. Genes with maturational gain in H2Bub1 and RNA expression were predominantly downregulated in RNF20/40 depletion (blue, upper-right quadrant), whereas genes with maturational loss in H2Bub1 and RNA expression were mostly upregulated in RNF20/40 depeletion (red, lower-left quadrant). g Ingenuity pathway analysis of functional terms enriched amongst the indicated genes. Left: Red-colored genes in the lower-left quadrant of panel f (i.e., genes that decrease H2Bub1 and gene expression during maturation and are upregulated in RNF20/40 depletion). Right: Blue-colored genes in the upper-right quadrant of panel f (i.e., genes that increase H2Bub1 and gene expression during maturation and are downregulated in RNF20/40 depletion). P-values were calculated by IPA Core Analysis. Source data are provided as a Source Data file.