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. 2021 Jul 21;11:14866. doi: 10.1038/s41598-021-94049-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of apoptosis-related proteins and ERK signaling. Cells were treated with solvent control (0.25% ethanol + 0.25% DMSO), 5-FU (75 µM), TNJ-Cr (0.25 mg/mL), TNJ-Bs (1.00 mg/mL), 5-FU (75 µM) + TNJ-Cr (0.25 mg/mL) and 5-FU (75 µM) + TNJ-Bs (1.00 mg/mL) for 48 h. Total ERK1/2 was used as a loading control (a). The relative fold of protein expression was shown as a bar graph from the intensity of the protein band compared with a loading control. Bar graph represents mean ± SD from three independent experiments (b). *p < 0.05 indicates a significant difference between the treatments and the solvent control; **p < 0.05 indicates a significant difference between the single and combined agent treatments. The blot images are shown in Supplementary Fig. S3.