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. 2021 Jul 21;12:4433. doi: 10.1038/s41467-021-24689-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of translational hp0102::gfpmut3 reporter fusion constructs originating from H. pylori strain 26695 (12G) that were integrated into the rdxA locus in H. pylori G27 wildtype and ΔrepG. The endogenous tlpB-hp0102 operon in G27 carries a 14G repeat. Red bar: tlpB promoter region; gray: G-repeat upstream of tlpB; black: tlpB coding region; orange: G-repeat upstream of hp0102; dark green: HP0102 coding region; light green: gfpmut3 coding sequence. Arrows indicate the transcriptional start site. b H. pylori G27 WT and ΔrepG strains that carry the indicated GFP reporter fusions were grown to exponential phase and HP0102::GFP protein levels were determined by western blot analysis. The relative HP0102::GFP protein levels in the ΔrepG mutant (vs. the respective wild-type background) are indicated by bars with corresponding s.d. (n = 6 biologically independent experiments; corresponding western blot representative is shown in Supplementary Fig. 3). ***—highly significant, p-value < 0.001; n.s.—not significant; Student’s t-test, two-tailed. Source data underlying (b) is provided as a Source data file.