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. 2021 Jul 21;7:95. doi: 10.1038/s41523-021-00305-w

Fig. 4. IL-1B signalling enables breast cancer metastasis initiation.

Fig. 4

a, b Tumour proliferation after injection of E0771 luc2 V5 IL1R1-GFP cells in IL1R1fl/fl (n = 8 primary tumours from n = 4 mice) and IL1R1−/− (n = 6 primary tumours from n = 3 mice) mice. Data are mean +/− SEM, Two-way ANOVA with Sidak’s multiple comparisons test. c In vitro relative tumour growth of E0771 luc2 V5 GFP, IL-1B GFP or IL1R1 GFP cells upon stimulation with 40 pg/ml mouse recombinant IL-1B. Data are mean (+/− SEM), Two-way ANOVA with Sidak’s multiple comparison test (n = 6 technical repeats from two biological replicates). d Transwell cell migration of IL-1B overexpressing (P = 0.0009) and IL1R1 overexpressing (P < 0.0001) E0771 luc2 V5 cancer cells compared to control GFP-expressing cells (no treatment with exogenous IL-1B). Comparison of cell migration in vitro between E0771 luc2 V5 IL-1B GFP and IL1R1 GFP tumour cells (P = 0.0018). Data are mean (+/− SEM) cell number from 4 fields of view derived from three biological experiments (n = 12 technical repeats). Two-tailed unpaired t-test with Welch’s correction. e Representative images of haematoxylin-stained control, IL-1B and IL-1R1 overexpressing cells migrated through the membrane. Scale bar = 200 µm.