Figure 4.

Pretreatment of hBMSC-derived exosomes reduced production of iROS in hTMCs when exposed to H₂O₂. HTMCs were pretreated with hBMSC-derived exosomes or PBS for 24 h, then exposed to H₂O₂ (0.1 mM) for 6 h, and a blank control group cultured with medium containing exosome-free serum. (a) Intracellular ROS was identified with DCFDA staining and measured by flow cytometry. (b) Quantification of iROS fluorescence. n = 3 for each condition. A P value was obtained by a two-tailed unpaired t-test. *P < 0.05 compared to PBS + H₂O₂ group. Data were presented in Supplementary Table S3.