Figure 5.

Pretreatment of hBMSC-derived exosome down-regulated the expression of inflammatory factors in hTMCs when exposed to H₂O₂. HTMCs were pretreated with hBMSC-derived exosomes or PBS for 24 h, then exposed to H₂O₂ (0.1 mM) for 6 h and a blank control group cultured with medium containing exosome-free serum. Then, hTMCs were assayed by RT-PCR to assess mRNA expression of IL-1α (a), IL-1β (c), IL-6 (e), and IL-8 (g). Gene expression levels were normalized to GAPDH and presented as relative ratios. Total hTMC supernatants were analyzed for IL-1α (b), IL-1β (d), IL-6 (f), and IL-8 (h). n = 3 for each condition. A P value was obtained by a two-tailed unpaired t-test. *P < 0.05; **P < 0.01 compared with PBS + H₂O₂ group. Data were presented in Supplementary Tables S4 and S5.